Coding

Part:BBa_K2926056:Design

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


Fusion protein of Opy2 from yeast, mCherry and pVIII from the bacteriophage M13


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To enable proper protein folding we designed a short linker sequence coding for glycine and serine in between the three subdomains.


Source

The extracellular domain of Opy2 was synthesized via add on PCR, mCherry was obtained from the iGEM part BBa_J06504 and the major coat protein pVIII was excised from a helperphage plasmid purchased from NEB.

References